[visionlist] anaesthesia

[Casagrande, Vivien]

Urethane is toxic and can’t be metabolized so can only be used in terminal surgeries as far as I know.

Vivien A. Casagrande, PhD
Professor
Departments of Cell & Developmental Biology, Psychology, Ophthalmology & Visual Sciences and Investigator Vanderbilt Kennedy Center

Office Address: T2302 Medical Center North
Mail Address:
Department of Cell & Developmental Biology
U3218 Learned Lab
Vanderbilt Medical School
Nashville, TN 37232-8240

Phone: 615-343-4538
Fax: 615-936-5673
Email: vivien.casagrande at vanderbilt.edu
http://www.psy.vanderbilt.edu/faculty/Casagrande/CasagrandeLab/index.htm

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From: visionlist-bounces at visionscience.com [mailto:visionlist-bounces at visionscience.com] On Behalf Of Agne Vaiceliunaite
Sent: Thursday, June 30, 2011 5:48 AM
To: visionlist at visionscience.com
Subject: [visionlist] anaesthesia

Hello,

I have a question about rats' anaesthesia for vision electrophysiology. For anaesthesia I decided to use Urethane. So far, I used 1000 mg/kg, i.p. 10% w/v in saline (P.Flecknell, Laboratory animal anaesthesia), but after that dosage, anaesthesia isn't so deep to perform craniotomy, so, additionally, I use i.p. perfusion 0,7ml/h. After surgery, even if I stop anaesthesia, rat dies in 6 hours. Maybe someone has any suggestions?

--
Sincerely,
Agnė Vaičeliūnaitė
___________________________
Department of Biochemistry and Biophysics
Faculty of Natural Sciences,
Vilnius University
Ciurlionio  21
03101 Vilnius, Lithuania
tel.: (+370 5) 2398218
fax: (+370 5) 2398216
email: agne.vaiceliunaite at gmail.com<mailto:agne.vaiceliunaite at gmail.com>
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